Articles
  • Design optimization of synthesis of composite calcium hydroxide loaded with ZnO and Ag nanoparticles as an antibacterial Agent

  • Mohsen Safaei,a,b Somaye Moloudi,c Razieh Souri,b Amir Sabzi,b Ling Shing Wongd and Roohollah Sharifib,e,*

  • aDivision of Dental Biomaterials, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran
    bAdvanced Dental Sciences and Technology Research Center, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran
    cStudents Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran
    dFaculty of Health and Life Sciences, INTI International University, Nilai 71800, Malaysia
    eDepartment of Endodontics, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran

  • This article is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Antibiotic resistance has become the major global health hazard that threatens people of all ages. The most important way to reduce infectious diseases in the treatment of the root canal is the elimination of infections caused by the accumulation of bacteria. The objective of this investigation was to enhance the production process of a newly developed nanocomposite composed of calcium hydroxide, zinc oxide, and silver. The intention behind this optimization was to achieve the utmost effectiveness in combating Enterococcus faecalis infection by exhibiting strong antibacterial properties. To fabricate nanocomposite with the best performance, the effects of three factors of calcium hydroxide (Ca(OH)2), zinc oxide (ZnO) and silver (Ag) nanoparticles (NPs), were investigated in three levels and nine different experiments designed using the Taguchi method. The successful formation of a nanocomposite with desired structural properties was confirmed through various analytical techniques including FTIR, UV-vis, XRD, SEM, EDX, X-Ray Map, TEM, and TGA. The findings indicated that the produced nanocomposite when subjected to the experimental circumstances of calcium hydroxide at a concentration of 50 mg/ml, ZnO at a concentration of 2 mg/ml, and Ag at a concentration of 2 mg/ml, exhibited the most potent antibacterial efficacy (0.26 CFU/ml) against the biofilm of Enterococcus faecalis. Due to the favorable antibacterial properties of this nanocomposite, its use as an in-channel drug can lead to more successful root canal treatments.


Keywords: Ceramic nanoparticles, Nanocomposite, Process innovation, Antibiotic resistance, Human health.

This Article

  • 2024; 25(4): 563-571

    Published on Aug 31, 2024

  • 10.36410/jcpr.2024.25.4.563
  • Received on Mar 17, 2024
  • Revised on Jun 5, 2024
  • Accepted on Jun 25, 2024

Correspondence to

  • Roohollah Sharifi

  • bAdvanced Dental Sciences and Technology Research Center, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran
    eDepartment of Endodontics, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran
    Tel : +98-833-37296591 Fax: +98-833-38277164

  • E-mail: roholahsharifi@gmail.com