Biphasic calcium phosphate (BCP) is a bioceramic that has two phases of tricalcium phosphate and hydroxyapatite which is inorganics composing bone. That has both osteoconductive and osteoinductive properties. Collagen is organic component composing bone. The aim of this paper is an assessment about membrane that was made with biphasic calcium phosphate and collagen. The specimens have various ratios of BCP/Collagen. The main production process is dry-freezing. It was undergone for 48 hrs. The specimens were evaluated for morphology, chemical behavior, cell cytotoxicity and animal test. The morphology was observed by scanning electron microscope. About the chemical behavior, specimens were measured using inductively coupled plasma optical emission spectrometer at 4 weeks and 8 weeks. Cell cytotoxicity was detected at 450 nm. For animal tests, rats were sacrificed at 12 weeks and 20 weeks.

Keywords: BCP, Collagen barrier, Cell cytotoxicity, Rats, Animal test.